In the present study, we find that OMT can significantly decrease the expressions of TLR3, TLR4, TLR7, MyD88, and TRAF6 genes after IAV infection, and we preliminarily speculate that OMT may inhibit IAV proliferation and IAV viral pneumonia via inhibiting TLRs signaling pathways. This evidence concerns the gene TRAF6 and viral pneumonia.