Western blot suggested that the level of expression of TNF-α in Group HF and Group ADR were higher than that of Group N (TNF-α: Group HF: 1.47 ± 0.14-fold of Group N, *p < .05; Group ADR: 2.47 ± 0.15-fold of Group N, *p < .05). The gene discussed is TNF; the disease is hydrops fetalis.