The results of real-time PCR and western blot (Figure 4(A–C)) revealed that high-fat diet induced the up-regulation of the TGF-β1 expression (real-time PCR: Group HF: 1.36 ± 0.13-fold of Group N, *p < .05; western blotting: Group HF: 1.47 ± 0.09-fold of Group N, *p < .05). This evidence concerns the gene TGFB1 and hydrops fetalis.