In multivariable logistic regression analysis, increased risk of anemia after 6 months of ART was observed among participants with age less than 30 years and CD4 count < 200 cells/mm3 whereas the odds of being anemic was lower among individuals with TDF based ART regimen, BMI 18.5–24.9 kg/m2 and male participants. The gene discussed is CD4; the disease is anemia.