TNNI3 and myocardial infarction: However, in low-risk patients (as defined by the Vancouver rule or a Thrombolysis in Myocardial Infarction Trial [TIMI] score of 0 or 1), the guidelines suggest that negative hs-cTnI at 0 and 2 h and negative hs-cTnI or hs-cTnT at 0 and 3–6 h may be used to rule out ACS (weak recommendation, low-quality evidence).