To further check whether modulation of chemotherapeutic outcome by obesity-associated factors is dependent on leptin or resistin, B16F10 and B16F1 cells were cultured in the medium containing HFD-C57BL/6J serum which was immuno-depleted of leptin or resistin by respective antibodies either alone or together. Here, RETN is linked to obesity due to melanocortin 4 receptor deficiency.