We previously described the construction of a functional humanized bsDb that targets EGFR and CD3 (hEx3-Db) [13] and enhanced its cytotoxicity by rearranging the domain order of the V domain, and also confirmed that cancer inhibitory effects against several cell lines including with low EGFR expression and in the case where unstimulated lymphocytes were applied as effector cells [14]. This evidence concerns the gene EGFR and cancer.