To evaluate the ability of meso-CART cells to target tumor cells expressing mesothelin, we constructed a lentiviral vector expressing full-length human mesothelin (Figure 2A) and examined its expression in several tumor cell lines using flow cytometry (Skov-3 human ovarian tumor and Panc-1, Aspc-1, and Capan-2 pancreatic cancer cell lines) (Figure 2B, black line: isotype, blue line: anti-mesothelin antibody). Here, MSLN is linked to familial pancreatic carcinoma.