To investigate TRIM52 expression and the effect of its ablation, U87MG glioblastoma cells were stably transduced with doxycycline (dox)-inducible lentiviral constructs encoding either non-targeting (NT) or TRIM52-targeting (T52) shRNAs in the artificial 3′-UTR of GFP [13] (Supplementary Figure 1A). This evidence concerns the gene TRIM52 and glioblastoma.