LDHA and colorectal cancer: To extrapolate our findings to additional colorectal cancer cell models, a panel of colorectal cancer cell lines: COLO320, HT29, HCT116, and SW620 were exposed to different concentrations of 5-FU for 5 days at a concentration of 6.2 and 12.5 μM and the percentages of ALDH+ cells were determined using the Aldefluor assay.