We applied the entire integrated workflow to sort and perform molecular analysis of rare CTCs from a preclinical model of pancreatic cancer (Pdx1-Cre, KrasLSL-G12D, p53R172H/+)50 with a yellow fluorescent protein (YFP) lineage tag (RosaYFP/YFP)), in which all pancreatic epithelial cells express YFP. This evidence concerns the gene PDX1 and pancreatic neoplasm.