Using time-lapse confocal microscopy we followed the migration trajectories of the GBM cell lines: GBM43 (primary GBM; mtp53, CDKN2A deletion), GBM10 (recurrent GBM derived from resection, received prior radiation and salvage chemotherapy; wildtype p53, CDKN2A deletion)[42–44], and the stem-like cell line GBAM1 (neurosphere forming, self-renewal, CD133+, SOX2+, Notch+, GFAP-)[31,45]. The gene discussed is GFAP; the disease is glioblastoma.