Although, the 3D coculture was modified for protein extraction, and small changes in signal transport could have existed between the two populations, the marked increase of GBAM1 STAT3 activation in presence of astrocytes (nearly 1.5-fold), suggests that GBAM1 shows a greater response to STAT3 activators expressed in presence of astrocytes compared to the other GBM lines studied. Here, STAT3 is linked to glioblastoma.