Survival assessment of the various GBM cell lines used in this study, showed that STAT3 inhibition by SH-4-54 was most effective in the stem-like cell line GBAM1 in 2D liquid culture, consistent with previously published work by Haftchenary et al (2013)[40] that shows reduced survival of brain tumor initiating cells after treatment with SH-4-54. The gene discussed is STAT3; the disease is brain neoplasm.