Migration analysis of GBM within a 3D in vitro tumor model showed that presence of astrocytes increases the migration of all GBM cell lines studied (GBM43, GBM10 and CD133+ GBAM1) while presence of ECFCs or ECFCs and astrocytes increases the total migration of GBM43 but not the total migration of GBAM1 (CD133+). This evidence concerns the gene PROM1 and glioblastoma.