In addition, stromal cells such as human astrocytes, the main non-neuronal stromal cell type of the brain[15] and human endothelial colony forming cells (ECFCs) were incorporated into the matrix and cultured with patient-derived GBM cell lines (GBM10 and GBM43) and the stem-like GBM line GBAM1 (CD133+). This evidence concerns the gene PROM1 and glioblastoma.