To demonstrate whether SIRT6 mediates these transcription factors in the chemosensitivity of HCC cells, we screened C/EBPβ, c-Jun, P53, and SP1 by using qPCR in SIRT6-depleted cells exposed to chemotherapeutic agents (Supplementary Figures 3B–D). This evidence concerns the gene TP53 and hepatocellular carcinoma.