Continued treatment with FSK and EGF following microcyst initiation caused maximal cyst formation and growth in NHK (64.3 ± 12.37 cysts/experiment with average cyst surface area of 18.5 ± 3.93 mm2) and ADPKD cells (83.0 ± 16.39 cysts/experiment with average cyst surface area of 46.6 ± 21.86 mm2) (Fig. 7; Figure S7), while treatment with Hh modulators alone did not influence cyst formation or growth (data not shown). The gene discussed is EGF; the disease is cyst.