Western blotting demonstrated a small increase (~1–2 fold) in the expression of total RNA-pol-II, CTD-S2P, CDK7, CDK9/P-TEFb, BRD4 and BRD2 in the 5-AZA-resistant M2AR and SCAR leukaemia cells compared to the original 5-AZA-sensitive OCI-M2 and SC leukaemia cells (Fig. 6a). This evidence concerns the gene BRD4 and leukemia.