In order to determine whether HDACi caused change in protein level of NIS, all of the thyroid cancer cell lines that are included in this study were incubated for 48 h with 10 nM panobinostat, 10 μM SAHA, and 1 μM Trichostatin A, and whole cell lysate was processed by western blot. Here, SLC5A5 is linked to thyroid cancer.