As negative control, KLK7 silenced hepatocellular carcinoma HepG2 cells were established, but our data showed that unlike PANC-1 cells, the knocking down of KLK7 did not affect the proliferation, migration, and invasion of HepG2 cells (Supplementary Figure 2), demonstrating that our designed LV-hKLK7-shRNA-4 do not target other proteins. This evidence concerns the gene KLK7 and hepatocellular carcinoma.