EMT is a process that increases the metastasis and invasive potential of tumor cells, with losing epithelial cell characteristics and acquiring a mesenchymal pheno-type, including decreasing epithelial markers (cytokeratin or E-cadherin) and up-regulating mesenchymal indicators (VIM or N-cadherin) or transcription factors (Twist, Snail) [35–37]. Here, TWIST1 is linked to neoplasm.