when lung cancer A549 cells with stable silencing Ang-2 transcription in the shRNA group, the level of the epithelial E-cadherin expression was significantly (P<0.001, Figure 4F) increasing than that in the NC or control group; otherwise the levels of mesenchymal VIM, and transcriptional factor Snail and Twist expressions were significantly (P<0.05, Figure 4G) decreasing than those in the NC or control group. This evidence concerns the gene TWIST1 and lung cancer.