TWIST1 and lung cancer: In the current study, the alterations of the EMT-related E-cadherin, VIM, Snail, and Twist level were analyzed (Figure 4) and showed that the A549 cells with stable silencing Ang-2 in the shRNA group, E-cadherin or signaling of VIM, Snail, and Twist were significantly increasing or decreasing than those in the NC or control group, indicated that abnormal Ang-2 expression promote lung cancer metastasis cascade through EMT formation.