The alterations of NPSR mRNA expression induced by PSD and the activation of neurons induced by NPS in the amygdala were analyzed using real-time qPCR and c-Fos ex-vivo immunohistochemistry (IHC) for revealing the potential mechanism of NPS-NPSR system involved in PSD-induced anxiety-like behavior and the effect of NPS on it. The gene discussed is FOS; the disease is Anxiety.