To further clarify the mechanism of anxiety-related behavior induced by 24-h PSD and identify the potential therapeutic targets, we determined the dynamic alteration of NPSR mRNA in the amygdala which is well known to play the key role in mediating anxiety and anxiolysis (Grupe and Nitschke, 2013; Tovote et al., 2015). The gene discussed is NPSR1; the disease is Anxiety.