We confirmed that the signatures associated with genes mutated in coding areas, including CDH1, MAP3K1, NEB, TAB3 and TP53, as well as the signatures related to genes with sequence variations in non-coding regions, such as AAK1, CA5A, FASTKD1, HUS1 and PDZD7, were predictive of OS in ER-positive/HER2-negative breast cancer (Supplementary Table 9). The gene discussed is AAK1; the disease is breast cancer.