Surprisingly, renal mRNA levels of the AKI marker lipocalin 2 (Lcn2; also known as neutrophil gelatinase–associated lipocalin [Ngal]) and kidney injury molecule-1 (Kim1, also named Tim1 and Havcr1), as well as renal mRNA levels of the pro–inflammatory cytokines interleukin (Il)–1 beta, Il–6 and tumor necrosis factor (Tnf) did not differ between sham or IR-injured kidneys of Trpv4 KO and WT mice (Fig. 2A–E). This evidence concerns the gene TNF and acute kidney injury.