Multi-variant analysis showed that higher expression of PFKFB3 was an independent prognostic factor for OS of HCC patients (hazard ratio (HR), 1.430; 95% confidence interval (CI), 1.204 to 1.997; p = 0.026) and DFS (HR, 1.426; 95% CI, 1.057 to 1.923; p = 0.05) (Table 2). The gene discussed is PFKFB3; the disease is hepatocellular carcinoma.