In our study, downregulation of WNT11, PRKCA and CaMK2D genes after TiO2 NPs treatment (10 μg/ml) possibly interfered with actin-cytoskeleton organization, cell movement and cell adhesion, thus disrupting noncanonical Wnt/Ca2+ signaling that resulted in omphalocele. Here, PRKCA is linked to omphalocele.