HIVEP2-DT and cystic fibrosis: In addition, we added 2 Italian isolates from patients with chronic obstructive pulmonary disease, 9 non-Italian clinical strains previously isolated from CF and non-CF patients, 5 environmental isolates, and 2 laboratory strains (PAO1 and PAO1 pilA) to increase the genetic diversity of the bacterial hosts tested (see Table S1 in the supplemental material).