H&E staining revealed that livers of Nrf2+/+ mice fed the high-calorie diet for 30 weeks, and given DMSO vehicle over the final 6 weeks, exhibited marked steatosis, inflammation, and hepatocyte ballooning (Figure 6C), which combined to give an average NAS of 4.9 (Figure 6D). This evidence concerns the gene NFE2L2 and steatosis.