Three neuronal populations enriched with tyrosine hydroxylase-positive (TH-positive) neurons differentiated from the iPSCs obtained via integrating method from a healthy donor (IPSRG2L) and two PD patients with the PARK8 gene mutation (IPSPDL1.6L and IPSPDL2.15L) were chosen for DNA methylation analysis. The gene discussed is LRRK2; the disease is Parkinson disease.