Finally, we conducted an immunohistochemistry assay using the xenograft tumor tissues, and found that the expression level of Ki-67 (a cellular proliferation marker) was decreased, but the autophagy marker, LC3B, was increased in the drug-resistant cells (Figure 3D, comparing lane 1 vs. lane 2, or lane 3 vs. lane 4). This evidence concerns the gene MKI67 and neoplasm.