In relation to this, we see choline-related annotations at all levels we analysed: our H3K27ac ChIP-seq analyses show changes in cholinergic synapse-related genes and differential binding related to Chrm2, the gene coding for M2; the KEGG annotation, ‘choline metabolism in cancer’, is enriched in our hippocampus RNA-seq genes and our H3K27ac ChIP-seq genes; finally, in our hippocampus RRBS, we see altered methylation of a CpG site within Ache, the gene coding for acetylcholinesterase. This evidence concerns the gene CHRM2 and cancer.