One of the putative genes was NTF3. Accordingly, we have investigated whether POU3F2 regulated NTF3 expression and the role of the POU3F2/NTF3 pathway in the early stages of neuronal development using a human pluripotent embryonic carcinoma cell line NTERA2 cl.D1 (NT2D1). The gene discussed is POU3F2; the disease is embryonal carcinoma.