By culturing HUVECs with high-glucose medium as a model mimicking microangiopathy in uncontrolled hyperglycemia of diabetes (Cagliero et al., 1991; Rubenstein et al., 2011; Patel et al., 2013), we demonstrated, for the first time, that CD40 was induced by high-glucose exposure. The gene discussed is CD40; the disease is Hyperglycemia.