We showed enhanced antitumor activity of evofosfamide (TH-302), a hypoxia-activated pro-drug selectively activated under low oxygen conditions to release the DNA cross-linker bromo-isophosphoramide mustard, in combination with pimasertib in human pancreatic cancer cell lines, an effect modulated through altered BRCA2 expression. This evidence concerns the gene BRCA2 and pancreatic neoplasm.