We performed SDV as a “preventative” measure in 3- to 5-month-old Mc4r−/− mice that had not yet attained full obesity (< 45 g) and as a “reversal” measure in 8-month-old Mc4r−/− mice that were severely obese (>50 g). The gene discussed is MC4R; the disease is obesity due to melanocortin 4 receptor deficiency.