On a stiff matrix when YAP is nuclear and co-localizes with PAX3 (Supplementary Figure S4), YAP depletion resulted in a significant decrease in MITF expression (Fig. 3g), but there was no contribution of YAP to MITF expression in melanoma cells grown on a 0.2 kPa soft matrix when the majority of YAP is cytoplasmic (Fig. 3g). This evidence concerns the gene MITF and melanoma.