Histological analysis showed that tumor-specific ablation of Notch1 did not change tumor morphology: indeed, ICC lesions were found throughout the liver and no hepatocellular tumors, including hepatocellular adenoma and HCC, were observed in both AKT/Yap/Cre and AKT/Yap/pCMV Notch1flox/flox mice (Fig. 4e). This evidence concerns the gene AKT1 and hepatocellular carcinoma.