Although targeting effects were demonstrated for all formats in vitro and stronger activity has been observed for EGFR-targeting scFvhu225-EHD2-scTRAIL compared to EHD2-scTRAIL in vivo, scFvhu225-Fc-scTRAIL and non-targeting Fc-scTRAIL showed similar potent anti-tumor effects in Colo205 xenograft models [28]. The gene discussed is EGFR; the disease is neoplasm.