The gene expression pattern also showed that 43 out of these 46 validated genes were upregulated and only three, collagen type VIII alpha 1 Chain (COL8A1), integrin subunit alpha 1 (ITGA1) and microfibril associated protein 5 (MFAP5), were downregulated in these breast cancer ECs compared with the control ECs (Figure 11). Here, COL8A1 is linked to breast carcinoma.