We next investigated the impact of PTT on induction of anti-tumor CD8+ T-cell responses by analyzing peripheral blood mononuclear cells (PBMCs) on day 7 after laser irradiation for the frequency of CD8+ T cells specific to the immune-dominant AH1 epitope (SPSYVYHQF), using AH1 peptide-major histocompatibility complex class I (H-2Ld) tetramer35. Here, CD8A is linked to neoplasm.