So we hypothesis that TRIP13 mediates EMT dependent on interacting with YWHAZ, knockdown of YWHAZ in TRIP13 overexpressing CRC cells and western blotting of E-cadherin, N-cadherin, β-catenin and snail were performed, the results showed that the up-regulation effect of N-cadherin, β-catenin, snail, and the down-regulation effect of E-cadherin induced by TRIP13 were rescued by YWHAZ knockdown, furthermore, we found that TRIP13 and YWHAZ were positively related. The gene discussed is TRIP13; the disease is colorectal carcinoma.