E2F7 and neoplasm: To determine the potential clinical relevance of the miR-30a-5p-regulated E2F7 gene in GBC specimens, we determined E2F7 expression levels in 42 GBC tumour tissue specimens and NATs by qRT-PCR, the results of which showed that E2F7 mRNA expression levels were significantly upregulated in the GBC tissue specimens compared with the NATs (Fig. 5e).