To investigate the impact of diabetes on platelet-endothelial adhesion, we established a mechanical endothelial injury model using micromanipulators and blunted microinjector needles (see Methods section and Supplementary Fig. 3a) that leads to the localized deposition of fibrinogen onto the surface of endothelial cells (Fig. 2a), which is dependent upon endothelial ICAM-1 binding (Fig. 2b). The gene discussed is ICAM1; the disease is diabetes mellitus.