TNF and neoplasm: Functional activity of the CAR was confirmed by demonstrating the ability of 4P28ζN+, but not 4PTrN+, T cells to elicit cytotoxicity (Fig. 2d) and release of interferon (IFN)-γ and tumor necrosis factor (TNF)-α (Fig. 2e), when co-cultivated with PLP but not PL tumor cells.