Two carcinoma datasets (breast and lung cancer) also displayed a universal E/M hybrid state with the co-expression of EPCAM and VIM. However, there also existed several differences in terms of the expression pattern of classical EMT-inducing TFs (Zeb1/2, Twist1/2, and Snai1/2): the hybrid state in lung cancer cells seemed to be more dependent on these TFs in comparison with that in breast cancer. This evidence concerns the gene TWIST1 and breast cancer.