Those cells are no reactive to anti-T-cruziantibodies, without any staining (Figure 1B).After infection and growth of T.cruzi amastigotes, those cellsexpress larger amounts of vimentin, with heavy staining of cytoplasm outside theparasitophorous vacuole and some particle shadowing patterns, suggesting thatvimentin are associated with cell cytoplasm (Figure1C). Here, VIM is linked to infection.