To investigate the role of TACSTD2 in regulating CLDN1 and OCLN distribution in hepatoma cells, we performed TACSTD2 gene silencing using a pool of 4 siRNA (siTACSTD2) and examined the distribution of the two HCV-entry cofactors by confocal microscopy. The gene discussed is TACSTD2; the disease is hepatocellular carcinoma.