One panel was the IonAmpliSeqTM Comprehensive Cancer Panel (Thermo Fisher Scientific) that covered the coding exons of 409 cancer-associated genes, and the other was an Ion AmpliSeqTM Custom DNA Panel (Thermo Fisher Scientific) that was designed to cover the coding exons of RHOA and its regulatory molecules, AKAP13 and DLC1 (Table 1). Here, RHOA is linked to cancer.