Using both primer set #1 and #2, we did not find evidence of ALK-I19 in the cDNA samples derived from neonatal brain, pooled neonatal human tissues or pooled adult human tissues; in addition, no evidence of ALK-I19 was found in a number of ALK-expressing, non-NB human cell lines including Karpas 299 and SupM2 (two ALK-positive anaplastic large cell lymphoma cell lines expressing NPM-ALK), H2228 (a lung cancer cell line expressing EML4-ALK) and U-87 MG (a glioblastoma cell line expressing wild-type full-length ALK)(Supplementary Figure 2A). This evidence concerns the gene ALK and glioblastoma.