This study represents a comprehensive analysis of the expression of the most discussed and most specific candidate stem cell (ABCG2, CD44, CD95, CD133, ELF4, Nanog, and Nestin) as well as differentiation and microglia markers (GFAP, Iba1, and Sparc) at the level of both mRNA and protein in primary GBM samples in comparison to nonmalignant brain specimens as well as in stem-like GBM neurospheres. This evidence concerns the gene NANOG and glioblastoma.