Briefly, using a series of diluted plasmid DNA as templates, we obtained a standard curve for FGFR2-IIIb and for FGFR2-IIIc, from which we obtained the exact copy number of both isoforms in ASCs, as well as in human fibroblasts (HFs), used as control for the mesenchymal lineage, and in the human breast cancer cell line MCF-7, used as control for the epithelial lineage (Figure 6(a)). The gene discussed is FGFR2; the disease is breast carcinoma.