Upon TNF-α treatment for short time (≤1 h), phosphorylation of IKKα and p65, and degradation of IκBα in CRC cells with either DDX27 overexpression or silencing were similar to that of control (Fig. S3A-S3D), indicating that regulation of TNF-α-induced NF-kB signaling by DDX27 is independent of IKK. The gene discussed is CHUK; the disease is colorectal carcinoma.