Interestingly, TNF-α treatment over extended periods (3–5 h) resulted in decreased expression of IκBα, and increased nuclear accumulation of p65 specifically in CRC cells overexpressing DDX27 (Fig. 5f and S3E), implying a possible positive autocrine feedback loop between activation of NF-кB and translocation of NF-кB p65. Here, NFKBIA is linked to colorectal carcinoma.