KIFC1 and neoplasm: Use of immunofluorescence-based scoring of the percentage cells with centrosome amplification that we used in cell lines may be impractical as a predictive biomarker for KIFC1 motor silencing in patient formalin-fixed paraffin-embedded (FFPE) tumor material, because individual cells cannot be assessed throughout their volume and centrosomes are frequently lost outside the plane of the histological section.