Accordingly, VEGF treatment enhanced endogenous CYR61 mRNA expression in a VEGFR-dependent manner in MCF10A-VEGFR1/2, MDA-MB231 breast cancer, human umbilical vein endothelial cell (HUVEC), an immortalized human endothelial cell line Telo-HEC, and human blood outgrowth endothelial cells (BOEC) (Fig. 4f, g and Supplementary Fig. 4a, b). This evidence concerns the gene FLT1 and breast cancer.